Composite

Part:BBa_K2423004:Design

Designed by: iGEM Uppsala 2017   Group: iGEM17_Uppsala   (2017-08-18)


Zeaxanthin to crocin enzymatic pathway


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1891
    Illegal NheI site found at 1914
    Illegal NheI site found at 3613
    Illegal NheI site found at 3636
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2079
    Illegal XhoI site found at 4199
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2486
    Illegal AgeI site found at 1774
    Illegal AgeI site found at 4372
    Illegal AgeI site found at 4727
    Illegal AgeI site found at 5878
    Illegal AgeI site found at 5990
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3003
    Illegal BsaI.rc site found at 672
    Illegal SapI.rc site found at 2111


Design Notes

We made complete devices including a promoter and RBS for each gene of the pathway. We also included a 6x his tag for easy purification of the expressed enzymes.


Source

Crocus ancyrensis, Crocus sativus

References